"In any cases, any allegations that have come forward to me, there has been a process in place and I have acted in accordance with respect to the need for confidentiality of people who have brought forward the allegations," she said.
Wynne's surprise disclosure came one day after federal Liberal Leader Justin Trudeau removed two MPs from his caucus amid allegations of inappropriate behaviour on Parliament Hill.
However, Wynne refused to say how many allegations were brought to her attention, or when, or if they involved elected Members of Provincial Parliament.
"I have taken action," she said at an unrelated event in Alliston, Ont. "I'm not going to go into specifics, but just know that I have taken action."
Some opposition members complained Wynne's comments could start an ugly guessing game at the legislature about just who may have been the subject of the harassment allegations.
Wynne again said the issue of sexual harassment in the workplace is not new, but declined to reveal any details of allegations she has had to deal with.
The premier has repeatedly rejected Progressive Conservative demands for an all-party committee of the legislature to study existing sexual harassment policies to see if they should be updated.