James Joseph Landry, 67, has pleaded not guilty to second-degree murder in the death of 43-year-old Philip Boudreau on June 1, 2013.
Three others, including members of Landry's family, also face charges in connection to the Boudreau case. Their trials have not been held yet.
Boudreau's overturned boat, a skiff, was found in Petit-de-Grat harbour, about 50 km east of Port Hawkesbury, but his body has never been recovered.
Last week the Crown told court that the crew shot at Boudreau four times, ran over his boat several times, and then tied his body to an anchor, which was dropped overboard.
The Crown has not elaborated yet on how the killing may have been tied to lobster.
The trial is being held in front of Nova Scotia Supreme Court Chief Justice Joseph Kennedy and a jury of 12 people.
On mobile? Follow the live blog here.