Thieves stole the Bible at night, first trying to remove a portion of the window using glass cutters, then when that didn't work, resorting to a smash and grab approach breaking the window and taking the book.
The Bible was the only thing taken, said Robyn Pyrczak, manager of the Bibles for Missions thrift shop on Victoria Road South.
"They obviously had it planned out," said Pyrczak.
Pyrczak didn't want to tell CBC News the value of the bible but said the starting bid was $250.
"Not only do we lose the money that could've been generated from the Bible, but also that window is going to cost us $1,000 to fix now," said Pyrczak.
The bible was donated to the Bibles for Missions store and is from 1850, with an embossed black leather cover and gold closures. It also includes colour illustrations by artist Gustave Dore.
Police are reviewing security footage from the store, Pyrczak said.
"We would like it to be in the hands of person who really would want it and know the true value of the words inside it," said Pyrczak.