The new image parallels Hubble's 1995 view of three columns of cold gas covered in ultraviolet light from a cluster of stars in a small area of the Eagle Nebula, which is 6,500 light years from Earth.
Inside the pillars, stars are being born, and the picture was aptly dubbed the Pillars of Creation.
The original image has been showcased on TV and in the movies, been printed on T-shirts and pillows and has graced a postage stamp.
The new image, which was taken with the telescope's wide field camera 3, offers a sharper and wider view of the pillars, and was taken in near-infrared and visible light. Newborn stars are pictured within the pillars.
The Hubble Space Telescope was launched aboard the Discovery shuttle in 1990.