The vaccination issue has returned to the forefront in recent weeks, with a spike in measles cases in Toronto and some parts of the United States.
During a news conference in Vancouver on Tuesday, Ambrose was asked for her thoughts on the movement that blames vaccines for a number of health issues.
"Vaccinations are, frankly, miracles of modern medicine. They are saving millions and millions of lives around the world, especially of children," she said.
"We're a country that is funding billions of dollars of vaccines for kids around the world, and here in Canada — where we have an educated population with a public health-care system, where your basic vaccinations are covered by the province — you have people who are refusing to vaccinate their children, putting them at risk.
A Queen's University professor is stepping aside from teaching a course students said included slides linking vaccinations to autism, while an Ottawa daycare offering a vaccine-free environment for children was criticized by the city's health department last week.