London Health Sciences Centre says in a statement that the pair were placed in a negative pressure room at the hospital just before 10 p.m. Tuesday.
The statement said the pair were not admitted through the emergency room and that "all safety precautions are fully in place to contain risk.”
The hospital has not released the names of the two patients and London Health Sciences Centre says there is "no impact to patient and visitor activity."
Earlier Tuesday, health officials said a patient at a Montreal hospital had tested negative for Ebola.
The regional health authority said the patient had begun exhibiting symptoms after returning from an Ebola-stricken part of Africa.