British Transport Police said officers were called to meet the train as it entered Stoke station, and asked four men to leave the train on Sunday night.
The force confirmed it followed "reports of racist and abusive behaviour by a number of passengers, said to be football fans."
It came hours after Chelsea won the League Cup final by beating Tottenham 2-0 at Wembley Stadium in north London.
Having spoken to two witnesses, police say investigations are continuing.
The behaviour of Chelsea fans has been in the spotlight after alleged racist incidents last month — one at a Paris metro station and one at London St. Pancras Station.