The Calgary Humane Society says a severely neglected bulldog cross was brought to an emergency veterinary clinic last September with facial and body wounds.
The dog was named Ruby by clinic staff and was emaciated with infections in her eyes and ears.
Ruby died from her injuries during surgery and humane society investigators asked the public to help identify her owner.
Tips eventually led investigators to search an address where 11 animals were seized in various states of neglect.
Janice Piper, Michelle Caswell and Jamie O’Leary are to appear in court March 31.
Piper and O’Leary are charged with respect to four animals, including Ruby. Caswell faces five counts with respect to seven animals.
"This was one of the worst cases of neglect we have seen," Brad Nichols, the humane society's senior manager of cruelty investigations, said in a statement Monday. "Ruby was suffering from so many serious issues.
"Ruby was an exceptionally sweet dog whose tail wagged to the very end, despite the pain that she was suffering."