Kate O'Brien and Monique Sullivan topped Cuba in 33.959 seconds in the women's team sprint to win the second gold medal of the day at the Cisco Milton Pan Am/Parapan Am Velodrome.
Cuba finished the head-to-head race in 34.813 while Colombia slipped past Mexico for bronze in 34.683.
Earlier, Hugo Barrette of Cap-aux-Meules, Que., Victoria's Evan Carey and Joseph Veloce of St. Catharines. Ont., edged Venezuela in the men's team sprint gold-medal race.
Canada finished three laps in 44.241 seconds.
Brazil edged Colombia for the bronze.