Foreign Affairs Minister Chrystia Freeland says in a statement that those people are now subject to an asset freeze and dealings prohibition.
Freeland says adding their names to the sanctions list is part of international pressure on the Assad regime to end indiscriminate violence against its own people, like this month's chemical weapons attack, and engage in meaningful negotiations.
Earlier this week, Freeland urged Russia, a longtime a supporter of Assad, to break with the Syrian president and help broker his departure in order to establish a lasting peace in the troubled region.
Minister of Foreign Affairs Chrystia Freeland responds to a question during question period in the House of Commons on Parliament Hill in Ottawa on Thursday.
Freeland says the new sanctions against key officials are part of Canada's continued efforts to pressure the Assad regime to stop the violence against innocent children, women and men.
The statement issued Friday says Canada is contributing to investigations on the use of chemical weapons and the collection of evidence to support the prosecution of war crimes and crimes against humanity in Syria.
"Last week's chemical weapons attack in southern Idlib is a war crime and is unacceptable,'' she said. "Canada is working with its allies to end the war in Syria and hold those responsible to account.''
Canada has committed $1.6 billion to efforts in the region to provide humanitarian, security, stabilization and development assistance, in addition to having welcomed more than 40,000 Syrian refugees to Canada.
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